Protein Name | Pulmonary Surfactant-Associated Protein BSp-B18 Kda Pulmonary-Surfactant Protein6 Kda ProteinPulmonary Surfactant-Associated Proteolipid Spl(Phe |
Database Links | Reactome: R-HSA-5683826Reactome: R-HSA-5688031Reactome: R-HSA-5688849Reactome: R-HSA-5688890 |
Cellular Localisation | SecretedExtracellular SpaceSurface Film |
Alternative ELISA Names | Pulmonary Surfactant-Associated Protein B ELISA kitSp-B ELISA kit18 Kda Pulmonary-Surfactant Protein ELISA kit6 Kda Protein ELISA kitPulmonary Surfactant-Associated Proteolipid Spl(Phe ELISA kitSFTPB ELISA kitSFTP3 ELISA kit |
Specificity | This kit recognizes Human SP-B in samples. No significant cross-reactivity or interference between Human SP-B and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 94-105 | 99 |
EDTA plasma (n=8) | 88-99 | 94 |
Cell culture media (n=8) | 89-101 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 2.42 | 7.23 | 22.89 | 2.26 | 7.33 | 20.64 |
Standard deviation | 0.16 | 0.35 | 0.71 | 0.12 | 0.41 | 1.05 |
CV (%) | 6.61 | 4.84 | 3.10 | 5.31 | 5.59 | 5.09 |