Post Translational Modifications | This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end. |
Function | Major acute phase protein. |
Protein Name | Serum Amyloid A-1 ProteinSaa Cleaved Into - Amyloid Protein AAmyloid Fibril Protein Aa - Serum Amyloid Protein A(2-104 - Serum Amyloid Protein A(3-104 - Serum Amyloid Protein A(2-103 - Serum Amyloid Protein A(2-102 - Serum Amyloid Protein A(4-101 |
Database Links | Reactome: R-HSA-3000471Reactome: R-HSA-416476Reactome: R-HSA-418594Reactome: R-HSA-444473Reactome: R-HSA-445989Reactome: R-HSA-6785807Reactome: R-HSA-879415Reactome: R-HSA-933542Reactome: R-HSA-977225 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Serum Amyloid A-1 Protein ELISA kitSaa Cleaved Into - Amyloid Protein A ELISA kitAmyloid Fibril Protein Aa - Serum Amyloid Protein A(2-104 - Serum Amyloid Protein A(3-104 - Serum Amyloid Protein A(2-103 - Serum Amyloid Protein A(2-102 - Serum Amyloid Protein A(4-101 ELISA kitSAA1 ELISA kit |
Specificity | This kit recognizes Human SAA in samples. No significant cross-reactivity or interference between Human SAA and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 84-95 | 90 |
EDTA plasma (n=8) | 89-100 | 94 |
Cell culture media (n=8) | 92-103 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 3.70 | 8.07 | 35.34 | 4.02 | 8.84 | 36.42 |
Standard deviation | 0.23 | 0.46 | 1.55 | 0.21 | 0.43 | 1.65 |
CV (%) | 6.22 | 5.70 | 4.39 | 5.22 | 4.86 | 4.53 |