Human RSPO3 protein (Recombinant-Active) (C-hFc-His) (STJP012245)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human RSPO3-C-hFc-His protein was developed from mammalian cells and has a target region of C-hFc-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8ยฐC for one week. Store at-20 to-80ยฐC for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to induce Topflash reporter activity in human embryonic kidney cells (HEK293T). The ED50 for this effect is 0.50โ2.00 ng/mL in the presence of 5 ng/mL recombinant Mouse Wntโ3a. |
Target Information
| Gene Symbol | RSPO3 |
| Gene ID | 84870 |
| Uniprot ID | RSPO3_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gln22-His272 |
Additional Info
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3. |
| Protein Name | R-Spondin-3Protein With Tsp Type-1 RepeatHpwtsrRoof Plate-Specific Spondin-3Hrspo3Thrombospondin Type-1 Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-4641263Reactome: R-HSA-8939256 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | R-Spondin-3 proteinProtein With Tsp Type-1 Repeat proteinHpwtsr proteinRoof Plate-Specific Spondin-3 proteinHrspo3 proteinThrombospondin Type-1 Domain-Containing Protein 2 proteinRSPO3 proteinPWTSR proteinTHSD2 protein |
Information sourced from Uniprot.org