Human RNF4 protein (Recombinant) (N-GST & C-His) (STJP016020)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RNF4-N-GST & C-His protein was developed from e.coli for the region N-GST & C-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | RNF4 |
| Gene ID | 6047 |
| Uniprot ID | RNF4_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Thr129-Ile190 |
Additional Info
| Post Translational Modifications | Sumoylated.conjugated by one or two SUMO1 moieties. Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation. Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf4Ring Finger Protein 4Small Nuclear Ring Finger ProteinProtein Snurf |
| Database Links | Reactome: R-HSA-5693607Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusPml Body |
| Alternative Protein Names | E3 Ubiquitin-Protein Ligase Rnf4 proteinRing Finger Protein 4 proteinSmall Nuclear Ring Finger Protein proteinProtein Snurf proteinRNF4 proteinSNURF proteinRES4-26 protein |
Information sourced from Uniprot.org