Human RNF34 protein (Recombinant) (His-Tag) (STJP018519)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human RNF34-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol 0.1M NaCl, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF34 |
| Gene ID | 80196 |
| Uniprot ID | RNF34_HUMAN |
| Accession Number | NP_919247 |
| Immunogen | Human |
| Immunogen Region | 1-373aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPE FSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLM RLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAP SATMSSFQGE LMDGDQTS |
Additional Info
| Tissue Specificity | Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. |
| Post Translational Modifications | Autoubiquitinated (in vitro). Proteolytically cleaved by caspases upon induction of apoptosis by TNF. |
| Function | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells. Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf34Caspase Regulator Carp1Caspases-8 And -10-Associated Ring Finger Protein 1Carp-1Fyve-Ring Finger Protein MomoHuman Ring Finger Homologous To Inhibitor Of Apoptosis ProteinHrfiRing Finger Protein 34Ring Finger Protein RiffRing-Type E3 Ubiquitin Transferase Rnf34 |
| Database Links | Reactome: R-HSA-6804757Reactome: R-HSA-983168 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinEndomembrane SystemNucleusNucleus SpeckleCytoplasmCytosol |
| Alternative Protein Names | E3 Ubiquitin-Protein Ligase Rnf34 proteinCaspase Regulator Carp1 proteinCaspases-8 And -10-Associated Ring Finger Protein 1 proteinCarp-1 proteinFyve-Ring Finger Protein Momo proteinHuman Ring Finger Homologous To Inhibitor Of Apoptosis Protein proteinHrfi proteinRing Finger Protein 34 proteinRing Finger Protein Riff proteinRing-Type E3 Ubiquitin Transferase Rnf34 proteinRNF34 protein |
Information sourced from Uniprot.org