Human RNA polymerase II subunit RPB4/POLR2D protein (Recombinant) (His-Tag) (STJP018369)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Rpolymerase II subunit RPB4/POLR2D-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Denatured |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLR2D |
| Gene ID | 5433 |
| Uniprot ID | RPB4_HUMAN |
| Accession Number | NP_004796 |
| Immunogen | Human |
| Immunogen Region | 1-142aa |
| Immunogen Sequence |
Additional Info
| Function | Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2D/RPB4 is part of a subcomplex with POLR2G/RPB7 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single-stranded DNA and RNA. |
| Protein Name | Dna-Directed Rna Polymerase Ii Subunit Rpb4Rna Polymerase Ii Subunit B4Dna-Directed Rna Polymerase Ii Subunit DRna Polymerase Ii 16 Kda SubunitRpb16 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-168325Reactome: R-HSA-203927Reactome: R-HSA-5578749Reactome: R-HSA-5601884Reactome: R-HSA-5617472Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-6803529Reactome: R-HSA-6807505Reactome: R-HSA-72086Reactome: R-HSA-72163Reactome: R-HSA-72165Reactome: R-HSA-72203Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075Reactome: R-HSA-8851708Reactome: R-HSA-9018519Reactome: R-HSA-9670095 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Dna-Directed Rna Polymerase Ii Subunit Rpb4 proteinRna Polymerase Ii Subunit B4 proteinDna-Directed Rna Polymerase Ii Subunit D proteinRna Polymerase Ii 16 Kda Subunit proteinRpb16 proteinPOLR2D protein |
Information sourced from Uniprot.org