• Biotinylated Human RGMa on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human RGMa is greater than 95% as determined by SEC-HPLC.

Human RGMa protein (Recombinant) (C-His-Avi) {Biotin} (STJP001731)

SKU:
STJP001731

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human RGMa-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: RGMA
Gene ID: 56963
Uniprot ID: RGMA_HUMAN
Immunogen Region: Cys48-Gly422
Immunogen: DNA sequence encoding Human RGMa including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Protein Name Repulsive Guidance Molecule A
Rgm Domain Family Member A
Database Links Reactome: R-HSA-373752
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Protein Names Repulsive Guidance Molecule A protein
Rgm Domain Family Member A protein
RGMA protein
RGM protein

Information sourced from Uniprot.org

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