Human RBPMS protein (Recombinant) (N-His-SUMO & C-Strep) (STJP008209)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RBPMS-N-His-SUMO & C-Strep protein was developed from e.coli for the region N-His-SUMO & C-Strep. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | RBPMS |
| Gene ID | 11030 |
| Uniprot ID | RBPMS_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Glu22-Glu97 |
Additional Info
| Function | Isoform A: RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets. Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state. Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats. Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes. Isoform C: Acts as a regulator of pre-mRNA alternative splicing (AS). Binds mRNA. Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA. Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins. |
| Protein Name | Rna-Binding Protein With Multiple SplicingRbp-MsRbpmsHeart And Rrm Expressed SequenceHermes |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmStress GranuleP-BodyLocalized To Cytoplasmic Stress Granules After Oxidative StressTranslocates Into Cytoplasmic Stress Granules That Probably Corresponds To P-Bodies In Response To Oxidative Stress |
| Alternative Protein Names | Rna-Binding Protein With Multiple Splicing proteinRbp-Ms proteinRbpms proteinHeart And Rrm Expressed Sequence proteinHermes proteinRBPMS proteinHERMES protein |
Information sourced from Uniprot.org