Human RAD51B protein (Recombinant) (His-Tag) (STJP018565)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP018565
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General Information

Short DescriptionRecombinant-Human RAD51B-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Denatured
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing, 10% Glycerol, 0.4M urea
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRAD51B
Gene ID5890
Uniprot IDRA51B_HUMAN
Accession NumberNP_002868
ImmunogenHuman
Immunogen Region1-350aa
Immunogen SequenceMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKG

Additional Info

Tissue Specificity Expressed in a wide range of tissues.
Post Translational Modifications Phosphorylated on tyrosine residues by BCR-ABL.
Function Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.
Protein Name Dna Repair Protein Rad51 Homolog 2
R51h2
Rad51 Homolog B
Rad51b
Rad51-Like Protein 1
Database Links Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693616
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709603
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Protein Names Dna Repair Protein Rad51 Homolog 2 protein
R51h2 protein
Rad51 Homolog B protein
Rad51b protein
Rad51-Like Protein 1 protein
RAD51B protein
RAD51L1 protein
REC2 protein

Information sourced from Uniprot.org

Citations

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