Human R-Spondin3 protein (Recombinant) (STJP000680)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding peptidase domain of Human R-Spondin3 (GLN22-VAL146) including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human R-Spondin3-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2ยฐC-8ยฐC C for one month, or at-20ยฐC to-70ยฐC for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). NA |
Target Information
| Gene Symbol | RSPO3 |
| Gene ID | 84870 |
| Uniprot ID | RSPO3_HUMAN |
| Immunogen | Optimized DNA sequence encoding peptidase domain of Human R-Spondin3 (GLN22-VAL146) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | GLN22-VAL146 |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. Highly expressed in endothelial cells. |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3. |
| Protein Name | R-Spondin-3Protein With Tsp Type-1 RepeatHpwtsrRoof Plate-Specific Spondin-3Hrspo3Thrombospondin Type-1 Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-4641263Reactome: R-HSA-8939256 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | R-Spondin-3 proteinProtein With Tsp Type-1 Repeat proteinHpwtsr proteinRoof Plate-Specific Spondin-3 proteinHrspo3 proteinThrombospondin Type-1 Domain-Containing Protein 2 proteinRSPO3 proteinPWTSR proteinTHSD2 protein |
Information sourced from Uniprot.org