Human R-spondin-2/RSPO2 protein (Recombinant) (C-hFc) (STJP003062)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human R-spondin-2/RSPO2 Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ala32-Gly205) of human R-spondin-2 (Accession #Q6UXX9) fused with hFc tag at the C-terminus.
General Information
| Short Description | Recombinant-Human R-spondin-2/RSPO2-C-hFc protein was developed in hek293 cells using the region Ala32-Gly205. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a Surrogate. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | RSPO2 |
| Gene ID | 340419 |
| Uniprot ID | RSPO2_HUMAN |
| Immunogen | Recombinant Human R-spondin-2/RSPO2 Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ala32-Gly205) of human R-spondin-2 (Accession #Q6UXX9) fused with hFc tag at the C-terminus. |
| Immunogen Region | Ala32-Gly205 |
| Immunogen Sequence | VTSAGPEESDGDLSCVCVKT ISSGIHLKHITSLEVIKAGR HCAVPQLIATLKNGRKICLD RQAPLYKKVIKKILES |
Additional Info
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form. |
| Protein Name | R-Spondin-2Roof Plate-Specific Spondin-2Hrspo2 |
| Database Links | Reactome: R-HSA-4641263 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | R-Spondin-2 proteinRoof Plate-Specific Spondin-2 proteinHrspo2 proteinRSPO2 proteinUNQ9384 proteinPRO34209 protein |
Information sourced from Uniprot.org