Human R-spondin-1 protein (Active Recombinant) (C-His) (STJP000847)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenActive Recombinant Human R-spondin-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Active Recombinant-Human R-spondin-1-C-His protein was developed in hek293 cells using the region Arg31-Ala263. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.|2.The intestinal crypts of mice were cultured in organoid cultu |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | RSPO1 |
| Gene ID | 284654 |
| Uniprot ID | RSPO1_HUMAN |
| Immunogen | Active Recombinant Human R-spondin-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Arg31-Ala263 |
| Immunogen Sequence | RISAEGSQACAKGCELCSEV NGCLKCSPKLFILLERNDIR QVGVCLPSCPPGYFDARNPD MNKCIKCKIEHCEACFSHNF CTKCKEGLYLHKGRCYPACP EGSSAANGTMECSSPAQCEM SEWSPWGPCSKKQQLCGFRR GSEERTRRVLHAPVGDHAAC SDTKETRRCTVRRVPCPEGQ KRRKGGQGRRENANRNLARK ESKEAGAGSRRRKGQQQQQQ QGTVGPLTSAGPA |
Additional Info
| Tissue Specificity | Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen. |
| Post Translational Modifications | C-, and N-glycosylated. N-glycosylation at Asn-137, negatively influences its secretion and enhancing effect on Wnt/beta-catenin signaling. C-mannosylation at Trp-156 by DPY19L3 is required for its secretion and regulates the enhancing activity of Wnt signaling. |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1. |
| Protein Name | R-Spondin-1Roof Plate-Specific Spondin-1Hrspo1 |
| Database Links | Reactome: R-HSA-4641263 |
| Cellular Localisation | SecretedNucleusSeems To Mainly Localize To Nucleoli |
| Alternative Protein Names | R-Spondin-1 proteinRoof Plate-Specific Spondin-1 proteinHrspo1 proteinRSPO1 protein |
Information sourced from Uniprot.org