| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description : | This PYY Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | PYY |
| Gene ID: | 5697 |
| Uniprot ID: | PYY_HUMAN |
| Specificity: | This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. |
| Protein Name | Peptide Yy Pyy Pyy-I Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 Pyy-Ii |
| Database Links | Reactome: R-HSA-375276 Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Peptide Yy ELISA kit Pyy ELISA kit Pyy-I ELISA kit Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 ELISA kit Pyy-Ii ELISA kit PYY ELISA kit |
| Specificity | This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-105 | 99 |
| EDTA plasma (n=8) | 85-100 | 91 |
| Cell culture media (n=8) | 89-103 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.33 | 303.30 | 971.46 | 107.16 | 318.05 | 955.53 |
| Standard deviation | 5.72 | 15.23 | 49.74 | 6.19 | 17.40 | 38.51 |
| CV (%) | 5.43 | 5.02 | 5.12 | 5.78 | 5.47 | 4.03 |