| Post Translational Modifications | Glycosylated. Four sites are completely N-glycosylated (Asn-640, Asn-731, Asn-865 and Asn-1425), whereas the others are found partially glycosylated. Processed by FURIN and the proteolytic processing largely depends on the peroxidase activity of PXDN. The proteolytic cleavage occurs after intracellular homotrimerization and releases into the extracellular matrix a large, catalytically active fragment and a smaller fragment consisting primarily of the C-terminal VWFC domain. The processing enhances both peroxidase activity and sulfilimine cross-links formation. |
| Function | Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1485' and leads to bromine enrichment of the basement membranes. In vitro, can also catalyze the two-electron oxidation of thiocyanate and iodide and these two substrates could effectively compete with bromide and thus inhibit the formation of sulfilimine bonds. Binds laminins. May play a role in the organization of eyeball structure and lens development during eye development. |
| Protein Name | Peroxidasin HomologMelanoma-Associated Antigen Mg50Peroxidasin 1Hspxd01Vascular Peroxidase 1P53-Responsive Gene 2 Protein Cleaved Into - Pxdn Active Fragment |
| Database Links | Reactome: R-HSA-2243919 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixEndoplasmic ReticulumCell SurfaceBasement MembraneEnriched In The Peritubular Space Of Fibrotic KidneysAdheres On The Cell Surface In 'Hot Spots'Only The Proteolytically Processed Pxdn Integrates Into The Extracellular MatrixPxdn Active Fragment: Secreted |
| Alternative Protein Names | Peroxidasin Homolog proteinMelanoma-Associated Antigen Mg50 proteinPeroxidasin 1 proteinHspxd01 proteinVascular Peroxidase 1 proteinP53-Responsive Gene 2 Protein Cleaved Into - Pxdn Active Fragment proteinPXDN proteinKIAA0230 proteinMG50 proteinPRG2 proteinPXD01 proteinVPO proteinVPO1 protein |
Information sourced from Uniprot.org
