Human PR3 (PR3) Antibody Pair (STJAP00906)
General Information
| Short Description | This PR3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R01-8G-9/R02-2B-2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PRTN3 |
| Gene ID | 5657 |
| Uniprot ID | PRTN3_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in polymorphonuclear leukocytes (at protein level). Expressed in neutrophils (at protein level). Expressed in differentiating neutrophils. |
| Function | Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. Plays a role in neutrophil transendothelial migration, probably when associated with CD177. Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation. |
| Protein Name | MyeloblastinAgp7C-Anca AntigenLeukocyte Proteinase 3Pr-3Pr3Neutrophil Proteinase 4Np-4P29Wegener Autoantigen |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-449836Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | Cytoplasmic GranuleSecretedCell MembranePeripheral Membrane ProteinExtracellular SideMembrane RaftLocalizes Predominantly To Azurophil Granules (Primary Secretory Granules) In NeutrophilsSecreted Upon Neutrophil Stimulation By Tnf-AlphaLipopolysaccharide (Lps)Fmlp And Cxcl8/Il8 Or During Neutrophil TransmigrationFollowing Secretion Tethered To The Cell Membrane By Cd177 |
| Alternative Antibody Pair Names | Myeloblastin antibody pairAgp7 antibody pairC-Anca Antigen antibody pairLeukocyte Proteinase 3 antibody pairPr-3 antibody pairPr3 antibody pairNeutrophil Proteinase 4 antibody pairNp-4 antibody pairP29 antibody pairWegener Autoantigen antibody pairPRTN3 antibody pairMBN antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PR3 (PR3) Antibody Pair (STJAP00906)
This antibody pair detects human Proteinase 3 (PR3), corresponding to UniProt entry P24158. Reactivity has been confirmed for human samples only.
Both antibodies in the pair are rabbit-derived monoclonal IgGs, with clone IDs R01-8G-9 and R02-2B-2. Both are supplied unconjugated and purified by affinity chromatography.
This antibody pair is validated for use in ELISA. It is intended for sandwich ELISA development, where one clone serves as the capture antibody and the other as the detection antibody.
Both antibodies are formulated in 0.01M PBS at pH 7.2. No carrier proteins, glycerol, or other additives are specified in the product details.
The optimal working concentration for each antibody is determined by the end user and should be established through titration during assay development. No predefined dilution range is specified for this product.
The antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.