| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human PPP2CA-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | PPP2CA |
| Gene ID: | 5515 |
| Uniprot ID: | PP2AA_HUMAN |
| Immunogen Region: | Met1-Leu309 |
| Immunogen: | Homo sapiens (Human) |
| Function | Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC.promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3.promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3. Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation. |
| Protein Name | Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha IsoformPp2a-AlphaReplication Protein CRp-C |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-1295596Reactome: R-HSA-141444Reactome: R-HSA-163685Reactome: R-HSA-163767Reactome: R-HSA-180024Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-198753Reactome: R-HSA-202670Reactome: R-HSA-2465910Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2995383Reactome: R-HSA-389513Reactome: R-HSA-432142Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5663220Reactome: R-HSA-5673000Reactome: R-HSA-5675221Reactome: R-HSA-6804757Reactome: R-HSA-6811558Reactome: R-HSA-68877Reactome: R-HSA-69231Reactome: R-HSA-69273Reactome: R-HSA-9634600Reactome: R-HSA-9648025Reactome: R-HSA-975957Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmNucleusChromosomeCentromereCytoskeletonSpindle PoleIn Prometaphase CellsBut Not In Anaphase CellsLocalizes At CentromeresDuring MitosisAlso Found At Spindle PolesCentromeric Localization Requires The Presence Of Sgo2 |
| Alternative Protein Names | Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform proteinPp2a-Alpha proteinReplication Protein C proteinRp-C proteinPPP2CA protein |
Information sourced from Uniprot.org
