| Host: | E.coli |
| Applications: | ELISA/Immunogen/SDS-PAGE/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human POLD1-N-GST protein was developed from e.coli and has a target region of N-GST. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | POLD1 |
| Gene ID: | 5424 |
| Uniprot ID: | DPOD1_HUMAN |
| Immunogen Region: | Met41-Phe254 |
| Immunogen: | Homo sapiens (Human) |
| Function | As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites. |
| Protein Name | Dna Polymerase Delta Catalytic Subunit3'-5' ExodeoxyribonucleaseDna Polymerase Subunit Delta P125 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-174411Reactome: R-HSA-174414Reactome: R-HSA-174417Reactome: R-HSA-174437Reactome: R-HSA-2564830Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183 |
| Cellular Localisation | NucleusColocalizes With Pcna And Pold3 At S Phase Replication SitesAfter Uv IrradiationRecruited To Dna Damage Sites Within 2 HoursIndependently On The Cell Cycle PhaseNor On Pcna UbiquitinationThis Recruitment Requires Pold3Pcna And Rfc1-Replication Factor C Complex |
| Alternative Protein Names | Dna Polymerase Delta Catalytic Subunit protein3'-5' Exodeoxyribonuclease proteinDna Polymerase Subunit Delta P125 proteinPOLD1 proteinPOLD protein |
Information sourced from Uniprot.org