| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.126ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This PICALM Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | PICALM |
| Gene ID: | 8301 |
| Uniprot ID: | PICAL_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in all tissues examined. |
| Post Translational Modifications | |
| Function | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). |
| Protein Name | Phosphatidylinositol-Binding Clathrin Assembly Protein Clathrin Assembly Lymphoid Myeloid Leukemia Protein |
| Database Links | Reactome: R-HSA-432722 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-9696264 |
| Cellular Localisation | Cell Membrane Membrane Clathrin-Coated Pit Golgi Apparatus Cytoplasmic Vesicle Clathrin-Coated Vesicle Nucleus Colocalized With Clathrin In The Golgi Area Interaction With Pimreg May Target Picalm To The Nucleus In Some Cells |
| Alternative ELISA Names | Phosphatidylinositol-Binding Clathrin Assembly Protein ELISA kit Clathrin Assembly Lymphoid Myeloid Leukemia Protein ELISA kit PICALM ELISA kit CALM ELISA kit |
| output |
Information sourced from Uniprot.org
