| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human PF4V1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PF4V1 |
| Gene ID: |
5197 |
| Uniprot ID: |
PF4V_HUMAN |
| Specificity: |
This kit recognizes Human PF4V1 in samples. No significant cross-reactivity or interference between Human PF4V1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74). |
| Function | Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). |
| Protein Name | Platelet Factor 4 VariantC-X-C Motif Chemokine 4 VariantCxcl4l1Pf4altPf4var1 Cleaved Into - Platelet Factor 4 Variant(4-74 - Platelet Factor 4 Variant(5-74 - Platelet Factor 4 Variant(6-74 |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-202733 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Platelet Factor 4 Variant CLIA kitC-X-C Motif Chemokine 4 Variant CLIA kitCxcl4l1 CLIA kitPf4alt CLIA kitPf4var1 Cleaved Into - Platelet Factor 4 Variant(4-74 - Platelet Factor 4 Variant(5-74 - Platelet Factor 4 Variant(6-74 CLIA kitPF4V1 CLIA kitCXCL4V1 CLIA kitSCYB4V1 CLIA kit |
| Specificity | This kit recognizes Human PF4V1 in samples. No significant cross-reactivity or interference between Human PF4V1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma(n=8) | 86-99 | 91 |
| Cell culture media(n=8) | 92-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.99 | 2.08 | 6.99 | 1.00 | 2.22 | 6.63 |
| Standard deviation | 0.12 | 0.22 | 0.65 | 0.08 | 0.22 | 0.63 |
| CV (%) | 12.12 | 10.58 | 9.30 | 8.00 | 9.91 | 9.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
