Human Peroxiredoxin 1/PRDX1 protein (Recombinant) (His-Tag) (STJP019740)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Peroxiredoxin 1/PRDX1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 7.5) containing 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is >2, 000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute. |
Target Information
| Gene Symbol | PRDX1 |
| Gene ID | 5052 |
| Uniprot ID | PRDX1_HUMAN |
| Accession Number | NP_002565.1 |
| Immunogen | Human |
| Immunogen Region | 1-199aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. Acetylation increases reducing activity and resistance to superoxidation. Deacetylated by HDAC6 which decreases reducing activity. The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner. |
| Function | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. |
| Protein Name | Peroxiredoxin-1Natural Killer Cell-Enhancing Factor ANkef-AProliferation-Associated Gene ProteinPagThioredoxin Peroxidase 2Thioredoxin-Dependent Peroxide Reductase 2Thioredoxin-Dependent Peroxiredoxin 1 |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-5628897Reactome: R-HSA-8862803Reactome: R-HSA-9818027 |
| Cellular Localisation | CytoplasmMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Protein Names | Peroxiredoxin-1 proteinNatural Killer Cell-Enhancing Factor A proteinNkef-A proteinProliferation-Associated Gene Protein proteinPag proteinThioredoxin Peroxidase 2 proteinThioredoxin-Dependent Peroxide Reductase 2 proteinThioredoxin-Dependent Peroxiredoxin 1 proteinPRDX1 proteinPAGA proteinPAGB proteinTDPX2 protein |
Information sourced from Uniprot.org