| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g |
| Immunoreactivity: | Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >19% cell adhesion. |
| Gene Symbol: | POSTN |
| Gene ID: | 10631 |
| Uniprot ID: | POSTN_HUMAN |
| Immunogen Region: | Asn22-Gln836 |
| Immunogen: | Recombinant Human Periostin/PN/POSTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn22-Gln836) of human Periostin/PN/POSTN (Accession #NP_006466.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | NNHYDKILAHSRIRGRDQGP NVCALQQILGTKKKYFSTCK NWYKKSICGQKTTVLYECCP GYMRMEGMKGCPAVLPIDHV YGTLGIVGATTTQRYSDASK LREEIEGKGSFTYFAPSNEA WDNLDSDIRRGLESNVNVEL LNALHSHMINKRMLTKDLKN GMIIPSMYNNLGLFINHYPN GVVTVNCARIIHGNQIATNG VVHVIDRVLTQIGTSIQDFI EAEDDLSSFRAAAITSDIL |
| Post Translational Modifications | Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. |
| Function | Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. |
| Protein Name | PeriostinPnOsteoblast-Specific Factor 2Osf-2 |
| Cellular Localisation | Golgi ApparatusSecretedExtracellular SpaceExtracellular MatrixColocalizes With Bmp1 In The Golgi |
| Alternative Protein Names | Periostin proteinPn proteinOsteoblast-Specific Factor 2 proteinOsf-2 proteinPOSTN proteinOSF2 protein |
Information sourced from Uniprot.org