Human PECAM1 (PECAM1) Antibody Pair (STJAP00962)
General Information
| Short Description | This PECAM1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20Β°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | PECAM1 |
| Gene ID | 5175 |
| Uniprot ID | PECA1_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
| Function | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. |
| Protein Name | Platelet Endothelial Cell Adhesion MoleculePecam-1EndocamGpiia'Peca1Cd Antigen Cd31 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-202733Reactome: R-HSA-210990Reactome: R-HSA-216083Reactome: R-HSA-432142Reactome: R-HSA-6798695Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated StimulationIsoform Long: Cell MembraneMembrane RaftCell JunctionLocalizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial CellsIsoform Delta15: Cell Junction |
| Alternative Antibody Pair Names | Platelet Endothelial Cell Adhesion Molecule antibody pairPecam-1 antibody pairEndocam antibody pairGpiia' antibody pairPeca1 antibody pairCd Antigen Cd31 antibody pairPECAM1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PECAM1 (PECAM1) Antibody Pair (STJAP00962)
This antibody pair detects human PECAM1 (CD31, UniProt P16284). Reactivity has been confirmed for human samples only.
The pair consists of two mouse monoclonal antibodies: the capture antibody is unconjugated and supplied at 0.5 mg/mL, while the detection antibody is biotinylated and supplied at a concentration of 0.5 to 4 ug/mL.
This antibody pair has been validated for use in sandwich ELISA with colorimetric detection. It has not been validated for other assay formats such as Western blot or immunohistochemistry.
This pair has been validated for use with serum, plasma, and other biological fluids. Compatibility with additional sample types such as cell culture supernatants or tissue lysates has not been confirmed in the provided product data.
A working dilution range of 1:1000 to 1:10000 is recommended. Optimal dilution should be determined empirically for each specific experimental setup.
Both components should be stored at -20Β°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.