| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. |
| Protein Name | Platelet-Derived Growth Factor Subunit BPdgf Subunit BPdgf-2Platelet-Derived Growth Factor B ChainPlatelet-Derived Growth Factor Beta PolypeptideProto-Oncogene C-SisBecaplermin |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1257604Reactome: R-HSA-186763Reactome: R-HSA-186797Reactome: R-HSA-2219530Reactome: R-HSA-3000171Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | SecretedReleased By Platelets Upon Wounding |
| Alternative ELISA Names | Platelet-Derived Growth Factor Subunit B ELISA kitPdgf Subunit B ELISA kitPdgf-2 ELISA kitPlatelet-Derived Growth Factor B Chain ELISA kitPlatelet-Derived Growth Factor Beta Polypeptide ELISA kitProto-Oncogene C-Sis ELISA kitBecaplermin ELISA kitPDGFB ELISA kitPDGF2 ELISA kitSIS ELISA kit |
| Specificity | This kit recognizes Human PDGF-BB in samples. No significant cross-reactivity or interference between Human PDGF-BB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 102 |
| EDTA plasma (n=8) | 85-101 | 92 |
| Cell culture media (n=8) | 87-100 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 108.61 | 262.35 | 849.02 | 105.51 | 269.64 | 922.05 |
| Standard deviation | 7.16 | 12.80 | 34.56 | 6.90 | 13.00 | 39.37 |
| CV (%) | 6.59 | 4.88 | 4.07 | 6.54 | 4.82 | 4.27 |
