• Biotinylated Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-PD-L1 Antibody, hFc Tag at 0. 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 2. 8ng/ml determined by ELISA.

Human PD-L1/B7-H1 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001643)

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STJP001643

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human PD-L1/B7-H1-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD274
Gene ID: 29126
Uniprot ID: PD1L1_HUMAN
Immunogen Region: Phe19-Arg238
Immunogen: DNA sequence encoding Human PD-L1/B7-H1 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation.
Function Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Protein Name Programmed Cell Death 1 Ligand 1
Pd-L1
Pdcd1 Ligand 1
Programmed Death Ligand 1
Hpd-L1
B7 Homolog 1
B7-H1
Cd Antigen Cd274
Database Links Reactome: R-HSA-389948
Reactome: R-HSA-9701898
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Early Endosome Membrane
Recycling Endosome Membrane
Nucleus
Associates With Cmtm6 At Recycling Endosomes
Where It Is Protected From Being Targeted For Lysosomal Degradation
Translocates To The Nucleus In Response To Hypoxia Via Its Interaction With Phosphorylated Stat3
Isoform 1: Cell Membrane
Isoform 2: Endomembrane System
Isoform 4: Secreted
Alternative Protein Names Programmed Cell Death 1 Ligand 1 protein
Pd-L1 protein
Pdcd1 Ligand 1 protein
Programmed Death Ligand 1 protein
Hpd-L1 protein
B7 Homolog 1 protein
B7-H1 protein
Cd Antigen Cd274 protein
CD274 protein
B7H1 protein
PDCD1L1 protein
PDCD1LG1 protein
PDL1 protein

Information sourced from Uniprot.org

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