Human ORM2 (Alpha-1-acid glycoprotein 2) Sandwich ELISA Kit (STJE0004098)

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STJE0004098
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description : This ORM2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ORM2
Gene ID: 5005
Uniprot ID: A1AG2_HUMAN
Specificity: This kit recognizes Human ORM2 in samples. No significant cross-reactivity or interference between Human ORM2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
Function Functions as a transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Protein Name Alpha-1-Acid Glycoprotein 2
Agp 2
Orosomucoid-2
Omd 2
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative ELISA Names Alpha-1-Acid Glycoprotein 2 ELISA kit
Agp 2 ELISA kit
Orosomucoid-2 ELISA kit
Omd 2 ELISA kit
ORM2 ELISA kit
AGP2 ELISA kit
Specificity This kit recognizes Human ORM2 in samples. No significant cross-reactivity or interference between Human ORM2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-108 100
EDTA plasma (n=8) 94-106 102
Cell culture media (n=8) 82-96 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.82 2.36 8.39 0.82 2.33 9.40
Standard deviation 0.04 0.11 0.36 0.04 0.11 0.45
CV (%) 4.88 4.66 4.29 4.88 4.72 4.79

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