Human OPN (Osteopontin) Antibody Pair (STJAP00168)
General Information
| Short Description | This OPN antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | SPP1 |
| Gene ID | 6696 |
| Uniprot ID | OSTP_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human OPN protein.Detection Immunogen: Recombinant Human OPN protein. |
Additional Info
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275Reactome: R-HSA-9856532 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Osteopontin antibody pairBone Sialoprotein 1 antibody pairNephropontin antibody pairSecreted Phosphoprotein 1 antibody pairSpp-1 antibody pairUrinary Stone Protein antibody pairUropontin antibody pairSPP1 antibody pairBNSP antibody pairOPN antibody pairPSEC0156 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human OPN (Osteopontin) Antibody Pair (STJAP00168)
This antibody pair detects human Osteopontin (OPN), corresponding to UniProt entry P10451. Reactivity has been confirmed for human samples only.
The capture antibody is an unconjugated mouse monoclonal antibody purified by Protein A or G, and the detection antibody is a biotin-conjugated goat polyclonal antibody purified by antigen affinity. Both components are supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4.
This pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human OPN protein. No specific immunogen region is defined in the current product data.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.