| Host: | Mouse/Goat |
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: | This OPN antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Clonality: | Monoclonal/Polyclonal |
| Conjugation: | Unconjugated/Biotin |
| Formulation: | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Purification: | Capture Purification: Protein A or G. Detection Purification: Antigen Affinity. |
| Concentration: | 0.5 mg/mL |
| Storage Instruction: | Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: | SPP1 |
| Gene ID: | 6696 |
| Uniprot ID: | OSTP_HUMAN |
| Immunogen: | Capture immunogen: Recombinant Human OPN protein. Detection Immunogen: Recombinant Human OPN protein. |
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | Osteopontin Bone Sialoprotein 1 Nephropontin Secreted Phosphoprotein 1 Spp-1 Urinary Stone Protein Uropontin |
| Database Links | Reactome: R-HSA-1474228 Reactome: R-HSA-186797 Reactome: R-HSA-216083 Reactome: R-HSA-381426 Reactome: R-HSA-8949275 Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Osteopontin antibody pair Bone Sialoprotein 1 antibody pair Nephropontin antibody pair Secreted Phosphoprotein 1 antibody pair Spp-1 antibody pair Urinary Stone Protein antibody pair Uropontin antibody pair SPP1 antibody pair BNSP antibody pair OPN antibody pair PSEC0156 antibody pair |
Information sourced from Uniprot.org
