| Host: | CHO cells |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Oncostatin M-protein was developed from cho cells. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered concentrated (1mg/ml) PBS solution, pH7.0. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The ED (50) was determined by the dose-dependent stimulation of the proliferation off human TF-1 cells is ≤3 ng/ml, corresponding to a specific activity of ≥2 x units/mg. NA |
| Gene Symbol: | OSM |
| Gene ID: | 5008 |
| Uniprot ID: | ONCM_HUMAN |
| Immunogen Region: | Mature chain |
| Immunogen: | Optimized DNA sequence encoding Human Oncostatin-M mature chain was expressed in CHO Cells NA |
| Immunogen Sequence: | MGVLLTQRTL LSLVLALLFP SMASMAAIGS CSKEYRVLLG QLQKQTDLMQ DTSRLLDPYI RIQGLDVPKL REHCRERPGA FPSEETLRGL GRRGFLQTLN ATLGCVLHRL ADLEQRLPKA QDLERSGLNI EDLEKLQMAR PNILGLRNNI YCMAQLLDNS DTAEPTKAGR GASQPPTPTP ASDAFQRKLE GCRFLHGYHR FMHSVGRVFS KWGESPNRSR RHSPHQAL |
| Post Translational Modifications | Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity. |
| Function | Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. |
| Protein Name | Oncostatin-MOsm |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-6788467 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Oncostatin-M proteinOsm proteinOSM protein |
Information sourced from Uniprot.org