| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description : | This human NT5E kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | NT5E |
| Gene ID: | 4907 |
| Uniprot ID: | 5NTD_HUMAN |
| Specificity: | This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. |
| Protein Name | 5'-Nucleotidase 5'-Nt 5'-Deoxynucleotidase Ecto-5'-Nucleotidase Imp-Specific 5'-Nucleotidase Thymidylate 5'-Phosphatase Cd Antigen Cd73 |
| Database Links | Reactome: R-HSA-196807 Reactome: R-HSA-73621 Reactome: R-HSA-74259 Reactome: R-HSA-9660826 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor |
| Alternative CLIA Names | 5'-Nucleotidase CLIA kit 5'-Nt CLIA kit 5'-Deoxynucleotidase CLIA kit Ecto-5'-Nucleotidase CLIA kit Imp-Specific 5'-Nucleotidase CLIA kit Thymidylate 5'-Phosphatase CLIA kit Cd Antigen Cd73 CLIA kit NT5E CLIA kit NT5 CLIA kit NTE CLIA kit |
| Specificity | This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-103 | 95 |
| EDTA plasma(n=8) | 92-107 | 98 |
| Cell culture media(n=8) | 85-97 | 91 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 199.21 | 595.70 | 1640.79 | 210.42 | 621.13 | 1523.54 |
| Standard deviation | 16.26 | 48.55 | 179.99 | 18.29 | 52.73 | 155.40 |
| CV (%) | 8.16 | 8.15 | 10.97 | 8.69 | 8.49 | 10.20 |