Post Translational Modifications | The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro). |
Function | NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. |
Protein Name | Pro-Neuropeptide Y Cleaved Into - Neuropeptide YNeuropeptide TyrosineNpy - C-Flanking Peptide Of NpyCpon |
Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418594Reactome: R-HSA-9615017 |
Cellular Localisation | SecretedCytoplasmic VesicleSecretory VesicleNeuronal Dense Core Vesicle |
Alternative ELISA Names | Pro-Neuropeptide Y Cleaved Into - Neuropeptide Y ELISA kitNeuropeptide Tyrosine ELISA kitNpy - C-Flanking Peptide Of Npy ELISA kitCpon ELISA kitNPY ELISA kit |
Specificity | This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-97 | 91 |
EDTA plasma(n=8) | 92-106 | 100 |
Cell culture media(n=8) | 87-99 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 99.80 | 187.00 | 734.30 | 100.00 | 174.50 | 759.20 |
Standard deviation | 6.60 | 8.20 | 25.00 | 6.20 | 7.70 | 29.60 |
CV (%) | 6.61 | 4.39 | 3.40 | 6.20 | 4.41 | 3.90 |