| Host: | E.coli |
| Applications: | ELISA/Immunogen/SDS-PAGE/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human NOS3-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | NOS3 |
| Gene ID: | 4846 |
| Uniprot ID: | NOS3_HUMAN |
| Immunogen Region: | Met1-Lys192 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| Protein Name | Nitric Oxide Synthase 3Constitutive NosCnosEc-NosNos Type IiiNosiiiNitric Oxide Synthase - EndothelialEndothelial NosEnos |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1474151Reactome: R-HSA-203615Reactome: R-HSA-203641Reactome: R-HSA-203754Reactome: R-HSA-392154Reactome: R-HSA-5218920Reactome: R-HSA-9009391Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneMembraneCaveolaCytoplasmCytoskeletonGolgi ApparatusSpecifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell CycleWhich Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative Protein Names | Nitric Oxide Synthase 3 proteinConstitutive Nos proteinCnos proteinEc-Nos proteinNos Type Iii proteinNosiii proteinNitric Oxide Synthase - Endothelial proteinEndothelial Nos proteinEnos proteinNOS3 protein |
Information sourced from Uniprot.org