Human NGF (Beta-nerve growth factor) Sandwich ELISA Kit (STJE0002794)
SPECIFICATIONS
General Information
| Short Description | This NGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 9.38pg/mL |
| Detection Limit | 15.63~1000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | NGF |
| Gene ID | 4803 |
| Uniprot ID | NGF_HUMAN |
| Specificity | This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Protein Name | Beta-Nerve Growth FactorBeta-Ngf |
| Database Links | Reactome: R-HSA-167021Reactome: R-HSA-167044Reactome: R-HSA-167060Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-177504Reactome: R-HSA-187042Reactome: R-HSA-187706Reactome: R-HSA-193648Reactome: R-HSA-193670Reactome: R-HSA-193681Reactome: R-HSA-198203Reactome: R-HSA-198745Reactome: R-HSA-205017Reactome: R-HSA-205025Reactome: R-HSA-205043Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-209563 |
| Cellular Localisation | SecretedEndosome LumenProngf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr |
| Alternative ELISA Names | Beta-Nerve Growth Factor ELISA kitBeta-Ngf ELISA kitNGF ELISA kitNGFB ELISA kit |
| Specificity | This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-99 | 93 |
| EDTA plasma (n=8) | 92-104 | 99 |
| Cell culture media (n=8) | 87-103 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.95 | 103.15 | 378.79 | 50.87 | 111.32 | 379.86 |
| Standard deviation | 2.77 | 4.32 | 14.39 | 2.93 | 6.37 | 15.92 |
| CV (%) | 5.66 | 4.19 | 3.80 | 5.76 | 5.72 | 4.19 |