| Short Description : | Recombinant-Human NEPH1-protein was developed from hek293. For use in research applications. |
| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability of immobilized recombinant human NEPH1 to bind mouse MS-1 endothelial cells, after 90 min 75% of cells will adhere. NA |
| Gene Symbol: | KIRREL1 |
| Gene ID: | 55243 |
| Uniprot ID: | KIRR1_HUMAN |
| Immunogen: | Optimized DNA sequence encoding extracellular domain of NEPH1 (GLN17-ALA499 ) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region: | GLN17-ALA499 |
| Tissue Specificity | Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. |
| Post Translational Modifications | Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding. N-glycosylated. |
| Function | Required for proper function of the glomerular filtration barrier. It is involved in the maintenance of a stable podocyte architecture with interdigitating foot processes connected by specialized cell-cell junctions, known as the slit diaphragm. It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. |
| Protein Name | Kin Of Irre-Like Protein 1Kin Of Irregular Chiasm-Like Protein 1Nephrin-Like Protein 1 |
| Database Links | Reactome: R-HSA-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPredominantly Located At Podocyte Slit Diaphragm |
| Alternative Protein Names | Kin Of Irre-Like Protein 1 proteinKin Of Irregular Chiasm-Like Protein 1 proteinNephrin-Like Protein 1 proteinKIRREL1 proteinKIRREL proteinNEPH1 protein |
Information sourced from Uniprot.org