| Post Translational Modifications | Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome. |
| Function | Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC.SGO1.NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction. Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation. Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells. |
| Protein Name | Serine/Threonine-Protein Kinase Nek2Hspk 21Never In Mitosis A-Related Kinase 2Nima-Related Protein Kinase 2Nima-Like Protein Kinase 1 |
| Database Links | Reactome: R-HSA-179409Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | Isoform 1: NucleusNucleusNucleolusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleChromosomeCentromereKinetochoreStk3/Mst2 And Sav1 Are Required For Its Targeting To The CentrosomeColocalizes With Sgo1 And Mad1l1 At The KinetochoreNot Associated With Kinetochore In The Interphase But Becomes Associated With It Upon The Breakdown Of The Nuclear EnvelopeHas A Nucleolar Targeting/ Retention Activity Via A Coiled-Coil Domain At The C-Terminal EndIsoform 2: CytoplasmPredominantly CytoplasmicIsoform 4: NucleusPredominantly Nuclear |
| Alternative Protein Names | Serine/Threonine-Protein Kinase Nek2 proteinHspk 21 proteinNever In Mitosis A-Related Kinase 2 proteinNima-Related Protein Kinase 2 proteinNima-Like Protein Kinase 1 proteinNEK2 proteinNEK2A proteinNLK1 protein |
Information sourced from Uniprot.org
