| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human MYL9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MYL9 |
| Gene ID: |
10398 |
| Uniprot ID: |
MYL9_HUMAN |
| Specificity: |
This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Smooth muscle tissues and in some, but not all, nonmuscle cells. |
| Post Translational Modifications | Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation. |
| Function | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation. |
| Protein Name | Myosin Regulatory Light Polypeptide 920 Kda Myosin Light ChainLc20Mlc-2cMyosin RlcMyosin Regulatory Light Chain 2 - Smooth Muscle IsoformMyosin Regulatory Light Chain 9Myosin Regulatory Light Chain Mrlc1 |
| Database Links | Reactome: R-HSA-3928663Reactome: R-HSA-416572Reactome: R-HSA-445355Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5627123Reactome: R-HSA-8936459 |
| Cellular Localisation | CytoplasmCytoskeletonCell CortexColocalizes With F-ActinMyh9 And Piezo1 At The Actomyosin Cortex In Myoblasts |
| Alternative CLIA Names | Myosin Regulatory Light Polypeptide 9 CLIA kit20 Kda Myosin Light Chain CLIA kitLc20 CLIA kitMlc-2c CLIA kitMyosin Rlc CLIA kitMyosin Regulatory Light Chain 2 - Smooth Muscle Isoform CLIA kitMyosin Regulatory Light Chain 9 CLIA kitMyosin Regulatory Light Chain Mrlc1 CLIA kitMYL9 CLIA kitMLC2 CLIA kitMRLC1 CLIA kitMYRL2 CLIA kit |
| Specificity | This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-108 | 100 |
| EDTA plasma(n=8) | 89-102 | 94 |
| Cell culture media(n=8) | 97-112 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.79 | 84.09 | 372.03 | 48.62 | 89.88 | 400.18 |
| Standard deviation | 5.96 | 6.25 | 27.12 | 5.84 | 8.39 | 38.10 |
| CV (%) | 11.51 | 7.43 | 7.29 | 12.01 | 9.33 | 9.52 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
