Human MSN protein (Recombinant) (N-GST) (STJP009986)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP009986
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General Information

Short DescriptionRecombinant-Human MSN-N-GST protein was developed from e.coli for the region N-GST. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolMSN
Gene ID4478
Uniprot IDMOES_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionMet1-Met577

Additional Info

Post Translational Modifications Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding. Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization. S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs. Modulates phagolysosomal biogenesis in macrophages. Also participates in immunologic synapse formation.
Protein Name Moesin
Membrane-Organizing Extension Spike Protein
Database Links Reactome: R-HSA-437239
Reactome: R-HSA-8950505
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Reactome: R-HSA-9725370
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Apical Cell Membrane
Cell Projection
Microvillus Membrane
Microvillus
Phosphorylated Form Is Enriched In Microvilli-Like Structures At Apical Membrane
Increased Cell Membrane Localization Of Both Phosphorylated And Non-Phosphorylated Forms Seen After Thrombin Treatment
Localizes At The Uropods Of T Lymphoblasts
Alternative Protein Names Moesin protein
Membrane-Organizing Extension Spike Protein protein
MSN protein

Information sourced from Uniprot.org

Citations

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