| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human MOG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MOG |
| Gene ID: |
4340 |
| Uniprot ID: |
MOG_HUMAN |
| Specificity: |
This kit recognizes Human MOG in samples. No significant cross-reactivity or interference between Human MOG and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes. |
| Function | Mediates homophilic cell-cell adhesion. Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. (Microbial infection) Acts as a receptor for rubella virus. |
| Protein Name | Myelin-Oligodendrocyte Glycoprotein |
| Cellular Localisation | Isoform 1: Cell MembraneMulti-Pass Membrane ProteinIsoform 5: Cell MembraneIsoform 2: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell MembraneIsoform 4: Cell MembraneIsoform 6: Cell MembraneIsoform 7: Cell MembraneIsoform 8: Cell MembraneIsoform 9: Cell Membrane |
| Alternative CLIA Names | Myelin-Oligodendrocyte Glycoprotein CLIA kitMOG CLIA kit |
| Specificity | This kit recognizes Human MOG in samples. No significant cross-reactivity or interference between Human MOG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-106 | 97 |
| EDTA plasma(n=8) | 101-117 | 107 |
| Cell culture media(n=8) | 89-102 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 109.28 | 321.29 | 863.05 | 106.61 | 301.04 | 799.73 |
| Standard deviation | 9.63 | 27.63 | 80.78 | 9.23 | 25.41 | 91.73 |
| CV (%) | 8.81 | 8.60 | 9.36 | 8.66 | 8.44 | 11.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
