Human MMP13 (MMP13) Antibody Pair (STJAP00786)
General Information
| Short Description | This MMP13 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R73-2Z-9/R78-6T-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MMP13 |
| Gene ID | 4322 |
| Uniprot ID | MMP13_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue. |
| Post Translational Modifications | The proenzyme is activated by removal of the propeptide.this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro). N-glycosylated. Tyrosine phosphorylated by PKDCC/VLK. |
| Function | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. |
| Protein Name | Collagenase 3Matrix Metalloproteinase-13Mmp-13 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-2022090Reactome: R-HSA-8941332 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Pair Names | Collagenase 3 antibody pairMatrix Metalloproteinase-13 antibody pairMmp-13 antibody pairMMP13 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human MMP13 (MMP13) Antibody Pair (STJAP00786)
This antibody pair is designed to detect human MMP13 (Matrix Metalloproteinase 13), corresponding to UniProt accession P45452. Reactivity has been confirmed for human samples only.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs at 1 mg/mL, formulated in 0.01M PBS at pH 7.2. Clone IDs are R73-2Z-9 and R78-6T-5 for the two components of the pair.
This antibody pair has been validated for use in ELISA. It is intended for sandwich ELISA development where one antibody serves as the capture reagent and the other as the detection antibody.
Optimal working concentrations are to be determined by the end user and are not pre-specified in the product data. Titration experiments are recommended to establish the appropriate dilutions for a given experimental setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both monoclonal antibodies in this pair were raised in rabbit against a recombinant protein immunogen corresponding to human MMP13. No further detail on the specific immunogen region is provided in the current product data.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.