| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human MMP-9-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: | MMP9 |
| Gene ID: | 4318 |
| Uniprot ID: | MMP9_HUMAN |
| Immunogen Region: | Ala20-Asp707 |
| Immunogen: | DNA sequence encoding Human MMP-9 (pro form) including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Post Translational Modifications | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. |
| Function | Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. |
| Protein Name | Matrix Metalloproteinase-9Mmp-992 Kda Gelatinase92 Kda Type Iv CollagenaseGelatinase BGelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 |
| Database Links | Reactome: R-HSA-1433557Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-2022090Reactome: R-HSA-3928665Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-9009391 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Matrix Metalloproteinase-9 proteinMmp-9 protein92 Kda Gelatinase protein92 Kda Type Iv Collagenase proteinGelatinase B proteinGelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 proteinMMP9 proteinCLG4B protein |
Information sourced from Uniprot.org
