Human MMP-1 protein (Recombinant) (C-His) (STJP000983)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human MMP1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe20-Asn469) of human MMP1 (Accession #NP_002412.1) fused with a 6ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Human MMP-1-C-His protein was developed in hek293 cells using the region Phe20-Asn469. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured in a cell migration assay using A549 cells. 1 ng/mL of Recombinant Human MMP-1 can effectively induce A549 cells migration.|2.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity i |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MMP1 |
| Gene ID | 4312 |
| Uniprot ID | MMP1_HUMAN |
| Immunogen | Recombinant Human MMP1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe20-Asn469) of human MMP1 (Accession #NP_002412.1) fused with a 6ΓHis tag at the C-terminus. |
| Immunogen Region | Phe20-Asn469 |
| Immunogen Sequence | FPATLETQEQDVDLVQKYLE KYYNLKNDGRQVEKRRNSGP VVEKLKQMQEFFGLKVTGKP DAETLKVMKQPRCGVPDVAQ FVLTEGNPRWEQTHLTYRIE NYTPDLPRADVDHAIEKAFQ LWSNVTPLTFTKVSEGQADI MISFVRGDHRDNSPFDGPGG NLAHAFQPGPGIGGDAHFDE DERWTNNFREYNLHRVAAHE LGHSLGLSHSTDIGALMYPS YTFSGDVQLAQDDIDGIQA |
Additional Info
| Post Translational Modifications | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. |
| Function | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. |
| Protein Name | Interstitial CollagenaseFibroblast CollagenaseMatrix Metalloproteinase-1Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-210991Reactome: R-HSA-381426Reactome: R-HSA-6785807 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Interstitial Collagenase proteinFibroblast Collagenase proteinMatrix Metalloproteinase-1 proteinMmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase proteinMMP1 proteinCLG protein |
Information sourced from Uniprot.org