| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human MICA Protein at 2 Mu g/mL (100 Mu L/well) can bind NKG2D with a linear range of 0.61-14.3 ng/mL. |
| Gene Symbol: |
MICA |
| Gene ID: |
100507436 |
| Uniprot ID: |
MICA_HUMAN |
| Immunogen Region: |
Met1-Gln308 |
| Immunogen: |
Recombinant Human MIC-A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gln308) of human MICA (Accession #NP_000238.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
MGLGPVFLLLAGIFPFAPPG AAAEPHSLRYNLTVLSWDGS VQSGFLTEVHLDGQPFLRCD RQKCRAKPQGQWAEDVLGNK TWDRETRDLTGNGKDLRMTL AHIKDQKEGLHSLQEIRVCE IHEDNSTRSSQHFYYDGELF LSQNLETKEWTMPQSSRAQT LAMNVRNFLKEDAMKTKTHY HAMHADCLQELRRYLKSGVV LRRTVPPMVNVTRSEASEGN ITVTCRASGFYPWNITLSW |
| Tissue Specificity | Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells. |
| Post Translational Modifications | N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol. N-glycosylation is necessary for cell surface expression. (Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation. |
| Function | Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production. |
| Protein Name | Mhc Class I Polypeptide-Related Sequence AMic-A |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmExpressed On The Cell Surface In Gastric EpitheliumEndothelial Cells And Fibroblasts And In The Cytoplasm In Keratinocytes And MonocytesInfection With Human Adenovirus 5 Suppresses Cell Surface Expression Due To The Adenoviral E3-19k Protein Which Causes Retention In The Endoplasmic Reticulum |
| Alternative Protein Names | Mhc Class I Polypeptide-Related Sequence A proteinMic-A proteinMICA proteinPERB11.1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
