Human MCAM/CD146 protein (Recombinant) (C-His) (STJP000877)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human MCAM/CD146 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Gly559) of human CD146/MCAM (Accession #NP_006491.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human MCAM/CD146-C-His protein was developed in hek293 cells using the region Val24-Gly559. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MCAM |
| Gene ID | 4162 |
| Uniprot ID | MUC18_HUMAN |
| Immunogen | Recombinant Human MCAM/CD146 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Gly559) of human CD146/MCAM (Accession #NP_006491.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Val24-Gly559 |
| Immunogen Sequence | VPGEAEQPAPELVEVEVGST ALLKCGLSQSQGNLSHVDWF SVHKEKRTLIFRVRQGQGQS EPGEYEQRLSLQDRGATLAL TQVTPQDERIFLCQGKRPRS QEYRIQLRVYKAPEEPNIQV NPLGIPVNSKEPEEVATCVG RNGYPIPQVIWYKNGRPLKE EKNRVHIQSSQTVESSGLYT LQSILKAQLVKEDKDAQFYC ELNYRLPSGNHMKESREVTV PVFYPTEKVWLEVEPVGML |
Additional Info
| Tissue Specificity | Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. |
| Function | Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. |
| Protein Name | Cell Surface Glycoprotein Muc18Cell Surface Glycoprotein P1h12Melanoma Cell Adhesion MoleculeMelanoma-Associated Antigen A32Melanoma-Associated Antigen Muc18S-Endo 1 Endothelial-Associated AntigenCd Antigen Cd146 |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013423Reactome: R-HSA-9035034 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Cell Surface Glycoprotein Muc18 proteinCell Surface Glycoprotein P1h12 proteinMelanoma Cell Adhesion Molecule proteinMelanoma-Associated Antigen A32 proteinMelanoma-Associated Antigen Muc18 proteinS-Endo 1 Endothelial-Associated Antigen proteinCd Antigen Cd146 proteinMCAM proteinMUC18 protein |
Information sourced from Uniprot.org