| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.061ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This MAPK13 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | MAPK13 |
| Gene ID: | 5603 |
| Uniprot ID: | MK13_HUMAN |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. |
| Post Translational Modifications | Dually phosphorylated on Thr-180 and Tyr-182 by MAP2K3/MKK3, MAP2K4/MKK4, MAP2K6/MKK6 and MAP2K7/MKK7, which activates the enzyme. Dephosphorylated by dual specificity phosphatase DUSP1. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells. |
| Protein Name | Mitogen-Activated Protein Kinase 13 Map Kinase 13 Mapk 13 Mitogen-Activated Protein Kinase P38 Delta Map Kinase P38 Delta Stress-Activated Protein Kinase 4 |
| Database Links | Reactome: R-HSA-168638 Reactome: R-HSA-171007 Reactome: R-HSA-4420097 |
| Cellular Localisation | |
| Alternative ELISA Names | Mitogen-Activated Protein Kinase 13 ELISA kit Map Kinase 13 ELISA kit Mapk 13 ELISA kit Mitogen-Activated Protein Kinase P38 Delta ELISA kit Map Kinase P38 Delta ELISA kit Stress-Activated Protein Kinase 4 ELISA kit MAPK13 ELISA kit PRKM13 ELISA kit SAPK4 ELISA kit |
| output |
Information sourced from Uniprot.org
