Human MANF/ARP protein (Recombinant-Active) (No tag) (STJP012033)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human MANF/ARP-No tag protein was developed from e.coli and has a target region of No tag. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to support the survival and stimulate neurite outgrowth ofrat embryonic cortical neurons. The ED50 for this effect is 0.7-2.8 Mu g/mL. |
Target Information
| Gene Symbol | MANF |
| Gene ID | 7873 |
| Uniprot ID | MANF_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Leu25-Leu182 |
Additional Info
| Function | Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange. As a result acts as a repressor of the unfolded protein response (UPR) pathway. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. Essential for embryonic lung development. Required for the correct postnatal temporal and structural development of splenic white pulp. Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling. Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury. Required for endochondral ossification in long bones and the skull during postnatal development. |
| Protein Name | Mesencephalic Astrocyte-Derived Neurotrophic FactorArginine-Rich ProteinProtein Armet |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | SecretedEndoplasmic Reticulum LumenSarcoplasmic Reticulum LumenRetained In The Endoplasmic Reticulum (Er)And Sarcoplasmic Reticulum (Sr) Under Normal ConditionsUp-Regulated And Secreted By The Er/Sr In Response To Er Stress And Hypoxia |
| Alternative Protein Names | Mesencephalic Astrocyte-Derived Neurotrophic Factor proteinArginine-Rich Protein proteinProtein Armet proteinMANF proteinARMET proteinARP protein |
Information sourced from Uniprot.org