| Host: | Insect cells |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human LYN-protein was developed from insect cells. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered 20mM Tris, 50mM NaCl, pH 8.0, 0.5mM PMSF. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was determined using a Glu/Tyr peptide substrate in a fluorogenic assay, and was determined to be 100nM/min/mg. NA |
| Gene Symbol: | LYN |
| Gene ID: | 4067 |
| Uniprot ID: | LYN_HUMAN |
| Immunogen Region: | ECD |
| Immunogen: | DNA sequence encoding extracellular domain of Human LYN to a N-terminal GST tag was expressed in Insect cells. NA |
| Tissue Specificity | Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. |
| Post Translational Modifications | Ubiquitinated by CBL, leading to its degradation. Ubiquitination is SH3-dependent. Autophosphorylated. Phosphorylated on tyrosine residues in response to KIT signaling. Phosphorylation at Tyr-397 is required for optimal activity. Phosphorylation at Tyr-508 inhibits kinase activity. Phosphorylated at Tyr-508 by CSK. Dephosphorylated by PTPRC/CD45. Becomes rapidly phosphorylated upon activation of the B-cell receptor and the immunoglobulin receptor FCGR1A. Phosphorylated in response to ITGB1 in B-cells. |
| Function | Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down-regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down-regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates phosphorylation of the BCR-ABL fusion protein. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3-kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr-72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation. Phosphorylates SCIMP on 'Tyr-107'.this enhances binding of SCIMP to TLR4, promoting the phosphorylation of TLR4, and a selective cytokine response to lipopolysaccharide in macrophages. Phosphorylates CLNK. Phosphorylates BCAR1/CAS and NEDD9/HEF1. |
| Protein Name | Tyrosine-Protein Kinase LynLck/Yes-Related Novel Protein Tyrosine KinaseV-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene HomologP53lynP56lyn |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-202733Reactome: R-HSA-2029481Reactome: R-HSA-210990Reactome: R-HSA-2454202Reactome: R-HSA-2682334Reactome: R-HSA-2730905Reactome: R-HSA-2871796Reactome: R-HSA-2871809Reactome: R-HSA-2871837Reactome: R-HSA-389356Reactome: R-HSA-389513Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-3928665Reactome: R-HSA-5621480Reactome: R-HSA-5621575Reactome: R-HSA-5690714Reactome: R-HSA-69231Reactome: R-HSA-75892Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284Reactome: R-HSA-912631Reactome: R-HSA-9664323Reactome: R-HSA-9664422Reactome: R-HSA-9670439Reactome: R-HSA-9674555Reactome: R-HSA-9680350Reactome: R-HSA-9705462Reactome: R-HSA-982772Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneNucleusCytoplasmPerinuclear RegionGolgi ApparatusMembraneLipid-AnchorAccumulates In The Nucleus By Inhibition Of Crm1-Mediated Nuclear ExportNuclear Accumulation Is Increased By Inhibition Of Its Kinase ActivityThe Trafficking From The Golgi Apparatus To The Plasma Membrane Occurs In A Kinase Domain-Dependent But Kinase Activity Independent Manner And Is Mediated By Exocytic Vesicular TransportDetected On Plasma Membrane Lipid Rafts |
| Alternative Protein Names | Tyrosine-Protein Kinase Lyn proteinLck/Yes-Related Novel Protein Tyrosine Kinase proteinV-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog proteinP53lyn proteinP56lyn proteinLYN proteinJTK8 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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