| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This LTF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
LTF |
| Gene ID: |
4057 |
| Uniprot ID: |
TRFL_HUMAN |
| Specificity: |
This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Isoform DeltaLf: Phosphorylation at Ser-10 activates the transcriptional activity. Phosphorylation at Ser-10 also promotes proteasomal degradation. Alternatively can undergo O-GlcNAcylation at Ser-10. Isoform DeltaLf: O-GlcNAcylation at Ser-10 inhibits DNA binding and negatively regulates the transcriptional activity. Alternatively can undergo phosphorylation at Ser-10. Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation. |
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin: Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity, which depends on the extracellular cation concentration. Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Can also prevent bacterial biofilm development in P.aeruginosa infection. Has weak antifungal activity against C.albicans. Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection. Can inhibit papillomavirus infections. Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling. Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells. Stimulates VEGFA-mediated endothelial cell migration and proliferation. Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA. Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection. N-terminal region shows strong antifungal activity against C.albicans. Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. Kaliocin-1: Has antimicrobial activity and is able to permeabilize different ions through liposomal membranes. Lactoferroxin-A: Has opioid antagonist activity. Shows preference for mu-receptor. Lactoferroxin-B: Has opioid antagonist activity. Shows higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferroxin-C: Has opioid antagonist activity. Shows higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites. Isoform DeltaLf: Transcription factor with antiproliferative properties and ability to induce cell cycle arrest. Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters. |
| Protein Name | LactotransferrinLactoferrinGrowth-Inhibiting Protein 12Talalactoferrin Cleaved Into - Lactoferricin-HLfcin-H - Kaliocin-1 - Lactoferroxin-A - Lactoferroxin-B - Lactoferroxin-C |
| Database Links | Reactome: R-HSA-1222449Reactome: R-HSA-6798695Reactome: R-HSA-6799990Reactome: R-HSA-6803157Reactome: R-HSA-977225 |
| Cellular Localisation | Isoform 1: SecretedCytoplasmic GranuleSecreted Into Most Exocrine Fluids By Various Endothelial CellsStored In The Secondary Granules Of NeutrophilsIsoform Deltalf: CytoplasmNucleusMainly Localized In The Cytoplasm |
| Alternative ELISA Names | Lactotransferrin ELISA kitLactoferrin ELISA kitGrowth-Inhibiting Protein 12 ELISA kitTalalactoferrin Cleaved Into - Lactoferricin-H ELISA kitLfcin-H - Kaliocin-1 - Lactoferroxin-A - Lactoferroxin-B - Lactoferroxin-C ELISA kitLTF ELISA kitGIG12 ELISA kitLF ELISA kit |
| Specificity | This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 91 |
| EDTA plasma (n=8) | 89-103 | 95 |
| Cell culture media (n=8) | 94-107 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.00 | 2.10 | 9.10 | 1.00 | 2.20 | 9.30 |
| Standard deviation | 0.10 | 0.10 | 0.40 | 0.10 | 0.10 | 0.40 |
| CV (%) | 10.00 | 4.76 | 4.40 | 10.00 | 4.55 | 4.30 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
