| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This LL-37 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CAMP |
| Gene ID: |
820 |
| Uniprot ID: |
CAMP_HUMAN |
| Specificity: |
This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The N-terminus is blocked. Proteolytically cleaved by proteinase PRTN3 into antibacterial peptide LL-37. Proteolytically cleaved by cathepsin CTSG and neutrophil elastase ELANE. Antibacterial peptide LL-37: Resistant to proteolytic degradation in solution, and when bound to both zwitterionic (mimicking mammalian membranes) and negatively charged membranes (mimicking bacterial membranes). After secretion onto the skin surface, the CAMP gene product is processed by a serine protease-dependent mechanism into multiple novel antimicrobial peptides distinct from and shorter than cathelicidin LL-37, such as peptides KR-20 (residues 151-170), LL-23 (residues 134-156), LL-29 (residues 134-162), KS-30 (residues 141-170), RK-31 (residues 140-170) and FF-33 (residues 138-170). The peptides act synergistically, killing bacteria at lower concentrations when present together, and maintain activity at increased salt condition. |
| Function | Antimicrobial protein that is an integral component of the innate immune system. Binds to bacterial lipopolysaccharides (LPS). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. Postsecretory processing generates multiple cathelicidin antimicrobial peptides with various lengths which act as a topical antimicrobial defense in sweat on skin. The unprocessed precursor form, cathelicidin antimicrobial peptide, inhibits the growth of Gram-negative E.coli and E.aerogenes with efficiencies comparable to that of the mature peptide LL-37 (in vitro). Antibacterial peptide LL-37: Antimicrobial peptide that is an integral component of the innate immune system. Binds to bacterial lipopolysaccharides (LPS). Causes membrane permeabilization by forming transmembrane pores (in vitro). Causes lysis of E.coli. Exhibits antimicrobial activity against Gram-negative bacteria such as P.aeruginosa, S.typhimurium, E.aerogenes, E.coli and P.syringae, Gram-positive bacteria such as L.monocytogenes, S.epidermidis, S.pyogenes and S.aureus, as well as vancomycin-resistant enterococci (in vitro). Exhibits antimicrobial activity against methicillin-resistant S.aureus, P.mirabilis, and C.albicans in low-salt media, but not in media containing 100 mM NaCl (in vitro). Forms chiral supramolecular assemblies with quinolone signal (PQS) molecules of P.aeruginosa, which may lead to interference of bacterial quorum signaling and perturbance of bacterial biofilm formation. May form supramolecular fiber-like assemblies on bacterial membranes. Induces cytokine and chemokine production as well as TNF/TNFA and CSF2/GMCSF production in normal human keratinocytes. Exhibits hemolytic activity against red blood cells. Antibacterial peptide FALL-39: Exhibits antimicrobial activity against E.coli and B.megaterium (in vitro). Antibacterial peptide KR-20: Acts synergistically with peptides KS-30 and KR-31, killing bacteria such as S.aureus, E.coli and C.albicans at lower concentrations when present together, and maintains activity at increased salt condition. Does not have the ability to stimulate CXCL8/IL8 release from keratinocytes. Antibacterial peptide LL-23: Poorly active (MIC > 150 uM) against E.coli strain K12. Is able to induce the pro-inflammatory cytokine TNF/TNFA or the chemokine CCL2/MCP1. Antibacterial peptide LL-29: Moderately antibacterial. Antibacterial peptide KS-30: Moderately antibacterial. Acts synergistically with peptides KR-20 and KR-31, killing bacteria such as S.aureus, E.coli and C.albicans at lower concentrations when present together, and maintain activity at increased salt condition. Does not have the ability to stimulate CXCL8/IL8 release from keratinocytes. Antibacterial peptide RK-31: Acts synergistically with peptides KS-30 and KR-31, killing bacteria such as S.aureus, E.coli and C.albicans at lower concentrations when present together, and maintain activity at increased salt condition. Does not have the ability to stimulate CXCL8/IL8 release from keratinocytes. Antibacterial peptide FF-33: Inhibits the growth of E.coli and B.megaterium and exhibits hemolytic activity against human red blood cells. |
| Protein Name | Cathelicidin Antimicrobial Peptide18 Kda Cationic Antimicrobial ProteinCap-18Hcap-18 Cleaved Into - Antibacterial Peptide Fall-39Fall-39 Peptide Antibiotic - Antibacterial Peptide Ll-37 - Antibacterial Peptide Kr-20 - Antibacterial Peptide Ll-23 - Antibacterial Peptide Ll-29 - Antibacterial Peptide Ks-30 - Antibacterial Peptide Rk-31 - Antibacterial Peptide Ff-33 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedVesicleStored As Pro-Peptide In Granules And Phagolysosomes Of NeutrophilsSecreted In Sweat Onto Skin |
| Alternative ELISA Names | Cathelicidin Antimicrobial Peptide ELISA kit18 Kda Cationic Antimicrobial Protein ELISA kitCap-18 ELISA kitHcap-18 Cleaved Into - Antibacterial Peptide Fall-39 ELISA kitFall-39 Peptide Antibiotic - Antibacterial Peptide Ll-37 - Antibacterial Peptide Kr-20 - Antibacterial Peptide Ll-23 - Antibacterial Peptide Ll-29 - Antibacterial Peptide Ks-30 - Antibacterial Peptide Rk-31 - Antibacterial Peptide Ff-33 ELISA kitCAMP ELISA kitCAP18 ELISA kitFALL39 ELISA kitHSD26 ELISA kit |
| Specificity | This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 101 |
| EDTA plasma (n=8) | 94-109 | 101 |
| Cell culture media (n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.72 | 12.01 | 44.23 | 4.99 | 11.72 | 45.29 |
| Standard deviation | 0.25 | 0.69 | 2.43 | 0.31 | 0.48 | 1.39 |
| CV (%) | 5.30 | 5.75 | 5.49 | 6.21 | 4.10 | 3.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
