• Biotinylated Human LAG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human LAG3 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-LAG3 Antibody, hFc Tag at 2 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12. 7ng/ml determined by ELISA.

Human LAG3/CD223 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001632)

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STJP001632

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human LAG3/CD223-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: LAG3
Gene ID: 3902
Uniprot ID: LAG3_HUMAN
Immunogen Region: Leu23-Gly434
Immunogen: DNA sequence encoding Human LAG3/CD223 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Lymphocyte activation gene 3 protein: Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner.
Function Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II).the precise role of MHC-II-binding is however unclear. Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.
Protein Name Lymphocyte Activation Gene 3 Protein
Lag-3
Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein
Slag-3
Database Links Reactome: R-HSA-2132295
Cellular Localisation Lymphocyte Activation Gene 3 Protein: Cell Membrane
Single-Pass Type I Membrane Protein
Secreted Lymphocyte Activation Gene 3 Protein: Secreted
Produced Following Cleavage Of The Main Chain
Alternative Protein Names Lymphocyte Activation Gene 3 Protein protein
Lag-3 protein
Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein protein
Slag-3 protein
LAG3 protein
FDC protein

Information sourced from Uniprot.org

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