| Post Translational Modifications | Phosphorylation by AURKB negatively regulates its interaction with protein phosphatase 1 (PP1) subunit PPP1CA and with microtubules. |
| Function | Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components.together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1. Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore. In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I. In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments. |
| Protein Name | Outer Kinetochore Knl1 Complex Subunit Knl1All1-Fused Gene From Chromosome 15q14 ProteinAf15q14Bub-Linking Kinetochore ProteinBlinkinCancer Susceptibility Candidate Gene 5 ProteinCancer/Testis Antigen 29Ct29Kinetochore Scaffold 1Kinetochore-Null Protein 1Protein Casc5Protein D40/Af15q14 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-606279Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreCytoplasmWeakly Expressed In Interphase NucleiExpression Increases From Prophase To Late AnaphaseBut Greatly Diminishes From The Telophase And Cytokinesis To Early G1 Phase Of Cell CycleLocalizes To The Cytoplasm During Meiotic Prophase I And Then The Nucleus As Meiosis Progresses |
| Alternative Protein Names | Outer Kinetochore Knl1 Complex Subunit Knl1 proteinAll1-Fused Gene From Chromosome 15q14 Protein proteinAf15q14 proteinBub-Linking Kinetochore Protein proteinBlinkin proteinCancer Susceptibility Candidate Gene 5 Protein proteinCancer/Testis Antigen 29 proteinCt29 proteinKinetochore Scaffold 1 proteinKinetochore-Null Protein 1 proteinProtein Casc5 proteinProtein D40/Af15q14 proteinKNL1 proteinCASC5 proteinKIAA1570 protein |
Information sourced from Uniprot.org
