Human KDM4A protein (Recombinant) (N-His) (STJP006547)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human KDM4A-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | KDM4A |
| Gene ID | 9682 |
| Uniprot ID | KDM4A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Tyr141-Lys310 |
Additional Info
| Post Translational Modifications | (Microbial infection) SUMOylated by human herpesvirus 8 E3 SUMO-protein ligase K-bZIP/K8 at Lys-471.thereby modulating the chromatin binding and histone demethylase activity of KDM4A. Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited. Also ubiquitinated by the SCF(FBXO22) complex.leading to proteasomal degradation. |
| Function | Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. |
| Protein Name | Lysine-Specific Demethylase 4aJmjc Domain-Containing Histone Demethylation Protein 3aJumonji Domain-Containing Protein 2aHistone H3-Trimethyl-L-Lysine(36 Demethylase 4aHistone H3-Trimethyl-L-Lysine(9 Demethylase 4a |
| Database Links | Reactome: R-HSA-3214842Reactome: R-HSA-5693565Reactome: R-HSA-9029569 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Lysine-Specific Demethylase 4a proteinJmjc Domain-Containing Histone Demethylation Protein 3a proteinJumonji Domain-Containing Protein 2a proteinHistone H3-Trimethyl-L-Lysine(36 Demethylase 4a proteinHistone H3-Trimethyl-L-Lysine(9 Demethylase 4a proteinKDM4A proteinJHDM3A proteinJMJD2 proteinJMJD2A proteinKIAA0677 protein |
Information sourced from Uniprot.org